Siddhartha Datta — новинки

Elimination of pollutants from contaminated sites using microorganisms is an economical alternative to chemical technology. A bacterium capable of utilizing Parathion as sole carbon and energy source isolated from agriculture effluent using selective enrichment on MS media containing Parathion, a commonly used insecticide. The strain was identified as belonging to the genus Cupriavidus based on a phylogram constructed. The experiment was studied under submerged culture in selective broth at varied interval of time in view of dry weight of bacterial biomass, further emphasizing the optimization of different process parameters by Central Composite Design and ANOVA for the bio-degradation of Parathion. The mathematical model fitting of the growth curve of the isolated bacteria is also shown. Towards the final phases, the chemical analysis, characterization of Parathion in effluent sample as well as the confirmation of its degradation is observed. The work reflects Cupriavidus sp. as potential degraders of the pesticide Parathion and highlights in utilizing the resourceful microbial world in treatment of agro-chemical effluent.

Phyto-chemicals are very significant, naturally acquiring chemical compounds present in the plants. It has wide application in nutraceuticals and preservation methods. Vigna radiata commonly known as Mung, is an undemanding and invasive plant species, able to grow and sustain in all environment. The ethano-medical value of Vigna radiata was investigated through phyto-chemical study and in vitro propagation methods. Phyto-chemical analysis quantified by GC-MS, showed the presence of flavonoids, carbohydrates, alkaloids, tannins, steroids, glycosides, and terpenoids. Since the intact plant contains low concentrations of active phyto-chemical compounds, plant cell cultures were employed as an alternative to produce large amounts of these metabolites. Moreover using elicitors the metabolite production can be increased. Elicitors like yeast extract and Aspergillus niger as biotic and CaCl2 as abiotic were used to stimulate the production of active phyto-chemical compounds. Furthermore, emphasizing the process optimization by Central Composite Design and ANOVA for percent increase in phytochemical content. Comparatively two fold increase of the plant height and two to ten times increase.